DNA Typing with 17 Microsatellites for Parentage Verification of Racehorses in Japan
نویسندگان
چکیده
منابع مشابه
Parentage Verification of Iranian Caspian Horse Using Microsatellites Markers
The present study was to construct a parentage verification system for Iranian Caspian horse. A total number of 45 Caspian horse samples including 14 foals for parentage verification, 17 stallion and 14 mare for individual identification were genotyped. Genomic DNA was extracted from whole blood and the genotype were analysed by PCR procedure and using 7 microsatellite markers (AHT04, HMS03, HM...
متن کاملparentage verification of iranian caspian horse using microsatellites markers
the present study was to construct a parentage verification system for iranian caspian horse. a total number of 45 caspian horse samples including 14 foals for parentage verification, 17 stallion and 14 mare for individual identification were genotyped. genomic dna was extracted from whole blood and the genotype were analysed by pcr procedure and using 7 microsatellite markers (aht04, hms03, hm...
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This study was performed for parentage verification of the Korean native horse (KNH). 103 random KNH samples (including 19 foals for parentage testing) were genotyped by using 16 microsatellite markers. The number of alleles per locus varied from 5 to 13 with an average value of 8.56 in the KNH. The observed heterozygosity and the expected heterozygosity ranged 0.3980.893 (the average value was...
متن کاملMicrosatellites in Portuguese autochthonous horse breeds: usefulness for parentage testing
The use of DNA technology for parentage testing is increasing every day. Most laboratories have improved their exclusion probabilities (PE) by the addition of DNA microsatellite loci to standard blood-typing results. The efficacy of each locus depends on the number of alleles detected and their frequencies in the breeds tested. Here we analyzed the usefulness of six microsatellites for routine ...
متن کاملValidation of 17 Microsatellite Markers for Parentage Verification and Identity Test in Chinese Holstein Cattle
To develop an efficient DNA typing system for Chinese Holstein cattle, 17 microsatellites, which were amplified in four fluorescent multiplex reactions and genotyped by two capillary electrophoresis injections, were evaluated for parentage verification and identity test. These markers were highly polymorphic with a mean of 8.35 alleles per locus and an average expected heterozygosity of 0.711 i...
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ژورنال
عنوان ژورنال: Nihon Chikusan Gakkaiho
سال: 2001
ISSN: 1346-907X,1880-8255
DOI: 10.2508/chikusan.72.453